AB

How do I use the new Smart Search feature to run a 2-Ab or 3-Ab panel?

To perform a 2-Ab query of ACTIN-SM and CYCLIN D1. Follow the steps for a single Ab search. [For instructions on running a single Ab query, please review the “Run a Single Ab Panel” tutorial.] Enter your first search term (i.e., “actin”) and select “ACTIN-SM” as Ab1. Notice that the word, “actin” is still highlighted in the search box. To enter the second antibody, simply start typing the common antibody name “cyclin” into the search box. The word “actin” will be replaced by the typed word.

Click the plusnext to “CYCLIN D1” or the name itself until it appears under the Selected ABS heading. Notice that once this antibody name has been selected a check appears next to the name in the place of the plus sign. In addition, the name “CYCLIN D1” appears under the Selected ABSheading. The info will display associated clone names for Selected ABS each selection. You may remove “CYCLIN D1” from the list by clicking the minus next to the name.

Click the red buildpanel button to run this 2-Ab query or continue reading for instructions on running a 3-Ab query.

To perform a 3-Ab query of ACTIN-SM, CYCLIN D1, and CD117. Follow the steps for a 2-Ab search. Two antibodies, “ACTIN-SM” and “CYCLIN D1” appear under the Selected ABS heading. Notice that the word “cyclin” is still highlighted in the search box. To enter the third antibody, simply start typing the common antibody class name “c-kit” into the search box. The word “cyclin” will be replaced by the typed word. [For ideas on effective keyword searches, please review our “Keyword Search” tutorial]

Click the plus next to “CD117” or the name itself until it appears under the Selected ABS heading. Notice that once this antibody name has been selected a check appears next to the name. The infowill display associated clone names of each selection.

Click thebuildpanel button to run this 3-Ab query.

What is the Ask an Expert Feature and how do I use it?

We have asked 13 acclaimed experts in their respective fields to tell us which antibodies they would run to confirm a diagnosis. The result is our new Ask an Expert feature. These customized panels offer at least 500 distinct two diagnosis and three diagnosis queries. Users compare our meta-analysis with what a world- renowned expert suggests, and ultimately improve patient care with increased diagnostic effectiveness.

In general, different bodies of knowledge are consulted when determining what antibodies will be used in a diagnosis. The Ask an Expert feature provides the pathologist with the first and only source for simultaneous viewing of empirical and evidentiary support to supplement their antibody decisions. A sample expert panel is shown below. The numbered and colored boxes correspond to a similarly numbered and colored paragraph description of each column. Briefly, the appearance of a symbol in the “Expert” column (boxes 1 and 3) indicates that a particular antibody is preferred by an expert. Plus (+) and minus (-) signs signify the reactivity attributed by the expert to a particular antibody and diagnosis combination. A yellow “caution” sign (shown in box 3) indicates special staining information or caveats attributed to that antibody/diagnosis combination. Expert information is juxtaposed with data from the meta-analysis (shown in boxes 2 and 4) for comparative purposes.

NOTE: The numbers and colored boxes in the picture correspond to the following numbered descriptions of each column of data:

1. The “Expert” column indicates with a plus (+) or minus (-) the preferred antibodies Dr. Moran uses to distinguish Lung Nonsmall Cell Carcinoma from Colon Adenocarcinoma. The + sign means that Dr. Moran attributes to this antibody a percent positivity of 60% or more for this diagnosis. A – sign represents a positivity of 40% or less. An equivocal reactivity (between 40% and 60%) is indicated by a “+/-” sign. These reactivities are assigned empirically by Dr. Moran and his lifetime of experience and may differ slightly from what is reported in the meta-analysis (shown in column 2).

2. All of the meta-analysis data for Lung Nonsmall Cell Carcinoma are available here in the “Positive,” “Cases,” and “vs.2” columns. The green arrows in the “vs.2” column indicate that, according to the meta-analysis, any of the first 4 antibodies would be appropriate to use for distinguishing Lung Nonsmall Cell Carcinoma from Colon Adenocarcinoma. Compare with Dr. Moran’s antibody selections. For these two differentials, the expert opinion and meta-analysis strongly agree.

3. The “Expert” column indicates with a + or – the preferred antibodies Dr. Greenson uses to distinguish Colon Adenocarcinoma from Lung Nonsmall Cell Carcinoma. The opposing Expert symbols across the “expert” columns indicate good differentiating antibodies for Lung Nonsmall Cell Carcinoma and Colon Adenocarcinoma (for example, CDX-2 and CK 20).

4. All of the meta-analysis data for Colon Adenocarcinoma are available here in the “Positive,” “Cases,” and “vs.2” columns. Comparing the “hard” numbers of the meta-analysis with the symbols in the expert column, one can see that the expert opinion strongly agrees with the meta-analysis.

How do I use the new Smart Search feature to run a single ab panel?

Perform a single Ab search of Smooth Muscle Actin. First, enter your first search term (i.e., “actin”). It can be a clone or class name. In the filtered list that is generated, find the desired antibody (shown in light blue, below). [For ideas on effective keyword searches, please review our Keyword Search post.]

To view the full alphanumeric list of antibody names in ImmunoQuery: if you prefer to search through the entire list of antibody names, click the View All link to the right of the search box. You will not need to enter any search terms into the text box.

Click the plusnext to “ACTIN-SM” or the name itself until it appears under the Selected ABS heading. To indicate that this antibody name has been selected, a check is shown next to the name.

In addition, the name “ACTIN-SM” appears under the Selected ABS heading. The infowill display associated antibody clone names the selection. You may remove “ACTIN-SM” from the selected ab list by clicking the minus next to the name.

Click thebuildpanel button to run this single antibody query.

[For information on running 2- and 3-ab queries, please review our “Run a 2-ab or 3-ab Panel” tutorial]

Elsevier